Weigert Van Gieson rapid method 100 test - cat# 04-053812 Bio Optica Special Stains

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Product for the preparation of cyto-histological samples for optical microscopy. To show elastic fibres, connective tissue and nuclei at the same time. Recommended for vascular pathology.

Method:

1) Bring section to distilled water.
2) Put on the section 5 drops of reagent A: leave to act 5 minutes.
3) Rinse in distilled water.
4) Prepare the incubation box: put on the bottom of the incubation box 20 drops of reagent B; introduce the slide and put on the section 10 drops of reagent C, close the incubation box and incubate 30 minutes.
5) Rinse in distilled water.
6) Put on the section 10 drops of reagent D: leave to act 2 minutes.
7) Wash the slide in tap water 5 minutes.
8) Rinse in distilled water.
9) Put on the section 5 drops of reagent E and add 5 drops of reagent F: leave to act 10 minutes.
10)Blue in running tap water 10 minutes.
11)Put on the section 10 drops of reagent G: leave to act 10 minutes.
12)Rinse quickly (2-3 seconds) in distilled water and dehydrate rapidly through ascending alcohols; stop 1 minute in the last absolute ethanol; clear in xylene and moun