Giemsa Pappenheim Staining Solution

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Primary container: white bottle in High Density Polyethylene (HDPE).

Expected aim: Product for the preparation of cyto-histological samples for optical microscopy.

Application: Reagent used with May Grünwald for the staining of different kind of cells in blood and bone marrow smears. This staining is also used to highlight the Helicobacter pylori in histology.
For the execution of the staining method is required the use of May Grünwald solution.

Method:

1) Air dry smears
2) May Gruenwald solution, 5 minutes
3) Wash in tap water, 1 minutes
4) Giemsa working solution*, 15 minutes
5) Wash in tap water, 1-2 minutes
6) Air dry

Results:

Nuclei : violet red purple
Lymphocyte cytoplasm: different blue tonalities
Monocyte cytoplasm: blue-grey
Eosinophil granulocytes (acidophil granules): brick red – orange
Basophil granulocytes (basophil granules): dark violet
Neutrophil granulocytes (neutrophil granules): pink-brown
Erythrocytes: pink –grey