Colloidal Iron 100 test - Special Stains

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Product for the preparation of cyto-histological samples for optical microscopy. For demonstration of the acid mucins. Specificity: the reaction shows the acid mucins (sialo and sulphured mucins) which form a stable complex with trivalent iron thanks to the presence of their acid groups in anionic form.

Method:

1) Bring section to distilled water.
2) Put on the section 10 drops of reagent A: leave to act 2 minutes.
3) Prepare the incubation box: put on the bottom of the incubation box 1 ml of distilled water; introduce the slide and put on the section 5 drops of reagent B and 5 drops of reagent C. Close the incubation box and incubate 1 hour.
4) Drain the slide without washing and put on the section 10 drops of reagent D: leave to act 1 minute. Drain and repeat the passage.
5) Drain the slide without washing and put on the section 10 drops of reagent E: leave to act 1 minute. Drain and repeat the passage.
6) Wash with distilled water.
7) Put on the section 10 drops of reagent F and 10 drops of reagent G: leave to act 20 minutes.
8) Wash well in distilled water.
9) Put on the section 10 drops of reagent H: leave to act 5 minutes.
10) Wash in distilled water.
11) Dehydrate through ascending alcohols, clear in xylene and mount