Bio-Agar - Intermediate medium for cell block processing - 10ml 15 vial

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Product for the preparation of cyto-histological samples for optical microscopy. Bio-Agar gel is an aggregation medium for cell block embedding procedure.

Fixation and Cytoembedding method:

Fixation:

1) To avoid the dispersion of cells and to abbreviate the procedure time, it is recommended to use, as collecting container, the 10 ml vials pre-filled with 5 ml neutral buffered formalin (Bio-Optica cod. 05- 01P05), which can be put directly into the centrifuge. Fixing time can takes from 15 minutes to some hours, according to the sample characteristics.

Cyto-embedding method:

1) Put a Bio-Agar vial into microwave oven to be melted at 160 Watt for 2 minutes. 2) Centrifuge the sample for 10 minutes at 2500 RPM. 3) Remove the exceeding liquid by a disposable pipette, avoiding the aspiration of eventual suspended fragments. 4) Add 6-10 drops of melted Bio-Agar. 5) Vortex immediately for some seconds. 6) Leave the sample to chill in the freezer (-20°C) until it solidifies - about 3 minutes - 7) Remove the sample pellet from the bottom of the vial and put it into a bio-cassette between two pads. 8) Process and embedd according to histologic routine.