Bielschowsky 100 test - Special Stains

Download Product's Datasheet

Product for the preparation of cyto-histological samples for optical microscopy. Recommended method to show neurofibrils, axons, dendrites and senile plaques in Alzheimer's disease.

Method:

1) Bring the section to distilled water.
2) Introduce the slide into the incubation box, put on the section 10 drops of reagent A. Close the incubation box and incubate 15 minutes at 40°C.
3) Extract the slide from the incubation box and wash well in distilled water.
4) Put again the slide back into the incubation box and put on the section 10 drops of reagent B. Close the incubation box and incubate 20 minutes at 50/55°C. During the incubation time, prepare the reducing solution: Introduce into a Coplin jar 50 ml of distilled water and add 10 drops of reagent C, 8 drops of reagent D, 8 drops of reagent E and 8 drops of reagent F. Shake briefly with a glass stick.
5) Without wash drain the slide and then put them into the reducing solution: incubate 1-2 minutes.
6) Double washing in distilled water.
7) Put on the section 10 drops of reagent G: leave to act 3 minutes.
8) Double washing in distilled water.
9) Dehydrate through ascending alcohols: clear in xylene and mount.